Plant DCL Proteins

Plant DCL ProteinsAbstractDicer, a double-stranded RNA (dsRNA)- special(prenominal) endoribonuclease, plays an necessary component part in triggering twain transcriptional and post-transcriptional gene silencing in eukaryotes by cleaving dsRNAs or single-stranded RNAs bearing nucleotide- twine conglomerate body parts such as microRNA harbinger transcripts into 21- to 24-nt micro RNAs. argumentationed animals, plants ware evolved to utilize at to the lowest degree quartet Dicer-like (DCL) proteins. Extensive transmitted studies kick in revealed that each DCL protein participates in a particular proposition gene silencing bridle-path, with close to redundancy. However, a mechanistic to a lower placestanding of how the specific action of each DCL protein is set in its respective pathway is still in its infancy overdue to the limited number of biochemical studies on plant DCL proteins. In this review, we re-start and discuss the biochemical properties of plant DCL pr oteins revealed by studies exploitation highly purified recombinant proteins, rocky sublimates, and immunoprecipitates. With help from co-factor proteins and an adenosine triphosphatease/DExH-box RNA-helicase landing field, the microRNA-producing enzyme DCL1 recognizes bulges and terminal loop structures in its substratum transcripts to ensure accurate and streamlined touch on. DCL4 prefers ache dsRNA substratums and requires the dsRNA-binding protein DRB4 for its application. The on the spur of the moment-dsRNA preference of DCL3 is well suited for short-RNA transcription and attendant dsRNA gaination by coupling between a plant-specific DNA- dependant RNA-polymerase IV and RNA-dependent RNA-polymerase 2 in the transcriptional gene silencing pathway. Inorganic phosphate overly seems to play a role in unlikeial regulation of DCL3 and DCL4 activities. Further development of biochemical approaches will be necessary for better understanding of how plant DCL proteins argon fine-tuned in each venial RNA biogeny pathway under various physiologic conditions.IntroductionRNA silencing, also know as RNA interference (RNAi), is unmatched of the fundamental molecular mechanisms keep in approximately eukaryotes to regulate gene expression both transcriptionally and post-transcriptionally. In both situations, what triggers the RNA silencing pathway is a smooth RNA molecule, 21 to 24 nt in continuance, called small interfering RNA (siRNA) or microRNA (miRNA) depending on its creation and the downstream pathways involved. The class 3 endoribonuclease (ribonucleinase) III enzymes known as Dicer argon responsible for producing siRNA from massiveer double-stranded RNAs (dsRNAs) and miRNA from single-stranded RNAs with internal bag-loop structures by a dsRNA-specific endoribonuclease. Therefore, the military action and regulation of Dicer-family proteins in a cell atomic number 18 vital to many an(prenominal) biological demonstratees requiring flexible adjustments at the level of gene expression, such as development, organogenesis, the circadian rhythm, biotic and abiotic stress responses, and defense against viruses and transposons.Biochemical characterization of Dicers in animalsThe Dicer family is a unique class of ribonucleinase III enzymes due to the strawman of an ATPase/DExD/H-box helicase field of honor at the N-terminus, a Piwi/Argonaute/Zwille (PAZ) farming in the middle and dual RNase III domains followed by one or devil dsRNA-binding domains in the C-terminal half (exception Giardia intestinalis) (Figure 1) (Bernstein et al. 2001). In general, the helicase domain serves as a protein-protein interaction summon recruiting co-factor regulative proteins (Lee et al. 2006 Ma et al. 2008 Ye et al. 2007). It also utilizes ATP hydrolysis to achieve processive cleavage of the hankersighted dsRNA substrate (Cenik et al. 2011 Welker et al. 2010). The PAZ domain view ass a maintain pocket for recognizing the terminus o f the dsRNA substrate, and the distance between PAZ and the RNase III catalytic center determines the product sizes (MacRae et al. 2007 MacRae et al. 2006). apiece of the deuce RNase III domains cuts one of the dsRNA strands, leaving a trace 2-nt overhang at 3-end of the product (Elbashir et al. 2001 Takeshita et al. 2007 Zhang et al. 2004). The C-terminal dsRNA-binding domains (dsRBDs) serve as a protein-protein interaction interface and nuclear localization signals, in addition to having dsRNA-binding bureau (Doyle et al. 2013 Hiraguri et al. 2005 Wostenberg et al. 2012). The specific functionality of each domain differs depending on the Dicer protein.Since the primary demonstration of in vitro small RNA-producing employment of Dicer in the fruit fly Drosophila melanogaster (Bernstein et al. 2001), its biochemical properties and regulatory machinery have been extensively studied in humans, D. melanogaster and Caenorhabditis elegans. In humans, there is besides one Dicer-fam ily protein (hDicer), which stupefys short-hairpin pre-miRNAs unveild by Drosha and dsRNA substrates into 20- to 22-nt small RNAs in an ATP-mugwump manner (Myers et al. 2003 Provost et al. 2002 Zhang et al. 2002). The cleavage use requires a divalent metal cation such as Mg2+, Co2+ or Mn2+, and recognizes mainly the 5-end of the substrate to ordain the product space (Park et al. 2011). This 5-counting rule is reliant on the conserved 3-pocket motif within the PAZ domain and the 5-pocket motif, which is less conserved in Dicers of new(prenominal) eukaryotes. The binding of Dicer to a dsRNA substrate and its cleavage atomic number 18 uncoupled, because Dicer hindquarters bind to dsRNA without Mg2+ or under low temperature (Provost et al. 2002 Zhang et al. 2002). The helicase domain of hDicer has an autoinhibitory function (Ma et al. 2008). In line with this, the action mechanism of recombinant full-duration hDicer protein rotter be ameliorate under limited proteolytic cond itions (Zhang et al. 2002).hDicer is responsible for both siRNA and miRNA return, and co-factor dsRNA-binding proteins TRBP and PACT dictate hDicer function in the two apparent small RNA production pathways (Chendrimada et al. 2005 Haase et al. 2005 Kok et al. 2007 Lee et al. 2013 Lee et al. 2006). In particular, the hDicer complex containing PACT disfavors siRNA precursor dsRNA and shows antithetical cleavage patterns on the same pre-miRNA substrate than the hDicer-TRBP complex (Lee et al. 2013). The interaction with TRBP occurs done the hDicer helicase domain, and stimulates the hDicers catalytic activeness. (Ma et al. 2008). Similarly, it has been reported that the C. elegans Dcr-1 interacts with a dsRNA-binding protein RDE-4 which enhances the Dicer activity toward foresightful dsRNA substrates in siRNA production, plot of ground RDE-4 is apparently dispensable in miRNA production pathway (Parker et al. 2006 Parker et al. 2008 Tabara et al. 2002).D. melanogaster has two D icer proteins, Dcr-1 and Dcr-2, which generate miRNA and siRNA, respectively (Lee et al. 2004 Miyoshi et al. 2010). Dcr-1 alone can process dsRNA into siRNA in vitro, yet its interaction with the dsRNA-binding protein Loquacious isoform PB (Loqs-PB) confers pre-miRNA substrate specificity to the Dcr-1-Loqs complex by destroying cleavage of long perfect dsRNAs and enhancing pre-miRNA affect activity (Saito et al. 2005 Zhou et al. 2009). Dcr-2 interacts with Loqs isoform PD and another dsRNA-binding protein, R2D2, in the siRNA production pathway (Liu et al. 2003 Liu et al. 2006 Miyoshi et al. 2010 Zhou et al. 2009). Dcr-2 alone is also capable of cleaving a pre-miRNA precursor in an ATP-independent manner, only if R2D2 significantly suppresses Dcr-2 activity toward pre-miRNA, whereas Loqs-PD enhances the cleavage activity of Dcr-2 toward long perfect dsRNA precursors by boosting its affinity to the substrate (Cenik et al. 2011 Miyoshi et al. 2010). The processive touch of long d sRNA substrates by Dcr-2 depends on ATP hydrolysis by its ATPase/helicase domain, implying that one of the functions of the helicase domain is to impart Dcr-2 to produce multiple siRNAs from a single long dsRNA molecule in front it dissociates from the substrate (Cenik et al. 2011). Such differential regulation of Dicer activity through specific interaction with co-factor dsRNA-binding proteins in distinct pathways is normally found in most of the establishments studied, including plants.DCL proteins in plantsPlant genomes contain at least four distinct classes of DCL family proteins (DCL1-4). Like their animal counterparts, each class of DCL has evolved to participate in its uncomplicated pathway (Figure 2), but the three siRNA-producing DCLs (DCL2-4) function redundantly as well, because defects in one class of DCL can be compensated for by other classes in some cases (Gasciolli et al. 2005 Mukherjee et al. 2013 Xie et al. 2004). Because DCL1 is the only Dicer protein that pro duces most 21-nt miRNAs (Kurihara and Watanabe 2004 Reinhart et al. 2002), knockout magnetic declinations of DCL1 are embryonic deadly (Schauer et al. 2002). DCL4 is the major producer of 21-nt antiviral siRNA and endogenous siRNAs such as trans-acting siRNA and phased siRNAs (phasiRNA) (Bouche et al. 2006 Gasciolli et al. 2005 Mukherjee et al. 2013 Qu et al. 2008 Xie et al. 2005 Yoshikawa et al. 2005). DCL2 can compensate for the loss of DCL4 (Bouche et al. 2006 Gasciolli et al. 2005 Parent et al. 2015), although its major function frame unclear. DCL3 mainly produces 24-nt repeat-associated siRNAs derived from transposons and DNA repetitive elements, and participates in transcriptional gene silencing (TGS) through RNA-dependent DNA methylation, suppressing proliferation of these elements (Henderson et al. 2006 Pontes et al. 2006 Xie et al. 2004). In addition to the four classes of DCLs, monocots have another distinct class of Dicer, DCL5 (also known as DCL3b) (Margis et al. 2006) . DCL5 is specifically denotative in developing panicles and is responsible for 24-nt fruitful phasiRNAs, although the biological significance of a reproductive-organ-specific 24-nt phasiRNA pathway intermediate by this specific Dicer remains to be elucidated (Borges and Martienssen 2015 Fei et al. 2013 Kapoor et al. 2008 Song et al. 2012). This pathway cogency be uniform to the Dicer-independent PIWI-interacting RNA (piRNA) pathway in vertebrates, which suppresses transposons and other genes specifically in glitchlines (Hirakata and Siomi 2016). both forward and reverse genetics and physiological studies have successfully cut the major RNA silencing pathways and allowed identification of the function of DCL genes in each pathway in plants. However, investigations on the molecular and enzymatic symptomatics underlying the functional variegation and specificity of the DCL proteins are still in their infancy.Detection of DCL activity in fossil oil draw ins of various plantsB iochemical characterization of plant Dicer activity was first exhibit in chaff germ take away (monocot) and cauliflower evoke (dicot), which contain multiple DCL activities producing 21 nt and 24 nt small RNAs with 2-nt 3-overhangs in the double-stranded form (Tang et al. 2003). These activities are weaker in the absence of ATP, consistent with characteristics of Dicer family proteins from Drosophila and C. elegans. Long dsRNA competitors effectively suppress both activities in wheat germ extract. The 24-nt small RNA producing activity was inhibited by 25-nt synthetic siRNA duplexes, whereas 21-nt small RNA production was untouched by 21-nt synthetic siRNA duplex competitors, suggesting that two different enzymes with active lays that have distinct size-dependent binding properties are in the wheat germ extract (Tang et al. 2003). A recent workplace on wheat germ extract characterized these activities in further detail, revealing (1) that the 21-nt activity could be found in a much bigger (950 kDa) complex than the 24-nt activity, which had maximum activity in an approximately 450 kDa complex and (2) the biochemical properties associated with the activities, such as divalent cation and NTP requirements, optimal NaCl concentration, temperature, and pH, and substrate length dependence (Shivaprasad et al. 2015). The identities of the DCL enzymes responsible for these activities in the wheat germ extract remain to be identified.A better understanding of the biochemical characteristics of individual plant Dicer proteins has come from the model plant genus Arabidopsis thaliana, which has four DCL proteins DCL1, DCL2, DCL3 and DCL4 (summarized in Table 1). The first in vitro DCL activity in A. thaliana was exhibit using a suspension cell lysate, a crude extract of florescence tissue, and an immunoaffinity-purified protein complex (Qi et al. 2005). Similar to the previous study using wheat germ extract or cauliflower, extracts from both Arabidopsis cultured cells and inflorescence tissue contained DCL dsRNA-cleaving activity producing 21- and 24-nt small RNAs from 400-bp dsRNA (Qi et al. 2005). The 21-nt producing activity and 24-nt producing activity were found in 660 kDa and 400 kDa fractions, respectively, suggesting that these Dicers reside in protein complexes composed of multiple co-factors (Qi et al. 2005).In proportionateness with previous genetic studies showing CARPEL FACTORY/DCL1 is responsible for 21-nt miRNA production in vivo (Kurihara and Watanabe 2004 Reinhart et al. 2002), the 21-nt small RNA producing activity was DCL1 immunoaffinity-purified from inflorescence-derived crude extract by an anti-DCL1 antibody (Qi et al. 2005). The 24-nt activity was associated with anti-DCL3 antibody immunoprecipitate, and the activity was repealed when purified from a dcl3-1 mutant, showing that DCL3 is responsible for the 24-nt activity in Arabidopsis inflorescence extract. The immunoaffinity-purified DCL1 activity required ATP, wh ereas the activity of the DCL3 immunoprecipitate was ATP-independent (Qi et al. 2005). Interestingly, the dcl1-7 edition did not abolish the 21-nt small RNA producing activity in the extract or immunoprecipitates, implying that the reversal (P415S) in its N-terminal helicase domain did not qualify the enzymes catalytic activity itself (Qi et al. 2005) this study also found that the activity of DCL4 responsible for formation of 21-nt siRNA was present in the inflorescence extract. The front line of DCL4 activity in an Arabidopsis crude extract was demonstrated in later studies using 2-week-old seedlings as the starting tangible (Fukudome et al. 2011 Nagano et al. 2014), and will be discussed later in this review.In-depth biochemical characterization of DCL1, a microRNA-producing enzyme in plantsDCL1 activity requires DRB1/HYL1 and SERRATE for accurate affect of the miRNA precursor twain in wheat germ and Arabidopsis extracts, DCL activities are associated with size fractions lar ger than DCL monomeric form, implying that these DCLs form functional protein complexes composed of multiple co-factors in vivo. As summarized in an earlier section, such interactions between a Dicer and a co-factor protein are commonly found in mammals, nematodes and insects. One of the most characterized classes of co-factor proteins is a dsRNA-binding protein (dsRBP) harboring multiple dsRNA-binding domains or motifs. The A. thaliana genome encodes five dsRNA-binding (DRB) family proteins DRB1/HYL1, DRB2 DRB3, DRB4, and DRB5. Multiple genetic and biochemical studies have demonstrated two specific interactions between DCLs and DRBs in A. thaliana DCL1-DRB1/HYL1 and DCL4-DRB4 (Han et al. 2004 Hiraguri et al. 2005 Kurihara et al. 2006 Nakazawa et al. 2007).Arabidopsis DCL1, DRB1/HYL1, and another co-factor, SERRATE (SE), create an essential microRNA production pathway in vivo (Han et al. 2004 Lobbes et al. 2006). hostile animals, which utilize two distinct RNase III enzymes, Drosh a and Dicer, for the first and indorsement cleavage of microRNA precursors, plants do not employ Drosha. Therefore, the DCL1-complex is responsible for the affect of both primary and precursor miRNA substrates. The detailed molecular machinery of the dual miRNA bear on mediated by DCL1 and the co-factor proteins have been extensively studied biochemically using highly purified recombinant proteins produced in heterologous systems (summarized in Figure 3). One of the systems utilizes baculovirus-mediated recombinant protein production in Sf21 insect cells, followed by two- tincture affinity purification (Dong et al. 2008). The highly purified recombinant DCL1 protein alone could process a 94-bp dsRNA substrate with a 2-nt 3-overhang into 21-nt small RNA in an ATP/Mg2+ dependent manner. The optimum NaCl concentration for the activity was 25-50 mM, and a NaCl concentration high than century mM severely impaired the activity (Dong et al. 2008). While the recombinant DCL1 protein alo ne could produce 21-nt small RNA from both primary and precursor miRNA (pri-/pre-miR167b) substrates in vitro, DRB1/HYL1 and SE recombinant proteins co-incubated in the same answer mixtures significantly increased both dedicate and accuracy of the bear upon (Dong et al. 2008). Without these co-factors, to a greater extent than 80% of 21-nt small RNA products from the DCL1-alone reaction were due to in catch up with processing from the end of the primary miRNA substrate, whereas the processing mediated by the DCL1-DRB1/HYL1-SE complex produced accurate 21-nt products with a sequence identical to miR167b/miR167b*, amounting for up to 81% of the products (Dong et al. 2008). This demonstrated that accurate processing of miRNA precursors by DCL1 requires the co-factors DRB1/HYL1 and SE. Consistent with a previous study, the interaction between DCL1-DRB1/HYL1 through the second dsRNA-binding motif of DCL1 is all important(p) for the precise processing of pri-miRNA in A. thaliana (Dong et al. 2008 Kurihara et al. 2006). Also, using highly purified recombinant proteins and surface plasmon ring analysis, it has been suggested that DCL1 changes its structural conformation when it binds RNA and exposes more binding sites for SE (Iwata et al. 2013). vertebral column to substrate dsRNA or miRNA precursors might be an important regulatory step for DCL1 dicing activity, as its dsRNA-binding domains exhibit the strongest binding to dsRNA among the four Arabidopsis DCLs (Hiraguri et al. 2005).ATPase/DExH-box RNA-helicase domain of DCL1 suppresses its dicing activity, confers ATP dependence, and diverges processing accuracyIn addition to its RNase III and dsRNA-binding domains, the helicase domain of DCL1 plays a significant role in regulating its dicing activity. Two independent forward genetic studies have identified two dcl1 mutant alleles, dcl1-13 (E395K) and dcl1-20 (R363K), as hyl1 suppressors, and the amino acid substitutions of both alleles occur within the ATPas e/DExH-box RNA-helicase domain. These dcl1 mutations partially hand over the accumulation of some miRNAs in a hyl1-2 mutant (Liu et al. 2012 Tagami et al. 2009), and dcl1-13 was at least partially able to restore the phenotypic defects of hyl1-2 such as a reduced number of rosette leaves and a leaf shape (Tagami et al. 2009). passing purified recombinant DCL1-20 protein exhibited enhanced catalytic activity (Kcat/Km) toward pri-miRNA156a compared to wild-type DCL1 (Liu et al. 2012). Similarly, the helicase domain-deleted DCL1 recombinant protein (DCL1Helicase) showed higher processing activity in vitro and was no long-lasting dependent on ATP for its activity toward pri-miRNA156a (Liu et al. 2012), suggesting that the helicase domain of DCL1 might have an autoinhibitory function like that of human Dicer (Ma et al. 2008 Provost et al. 2002).The in vivo miRNA processing imprecision in hyl1-2, however, was not restored by a dcl1-20 mutation, implying that the partial recuperation o f the hyl1-2 mutant, including miRNA accumulation, was due to the enhanced catalytic activity resulting from the substitution in the helicase domain (Liu et al. 2012). Interestingly, the effect and magnitude of DRB1/HYL1 and DCL1 helicase domain seem to vary among miRNA precursors. For example, the in vivo processing accuracy of miR156a is much more severely affected by hyl1-2 mutation than miR166b is (Liu et al. 2012). pri-miR156a is processed from the loop-proximal site to the loop-distal basis in vitro (Liu et al. 2012), which is considered unusual for plant miRNAs (Addo-Quaye et al. 2009 Mateos et al. 2010). immaculate processing of pri-miRNA166b by native DCL1 is largely dependent on the presence of ATP, and processing by DCL1Helicase is less accurate than that of native DCL1 (Liu et al. 2012). In contrast to miR156a, the processing precision of which is markedly affected by hyl1-2, that of miR166b was much more impaired by dcl1-20 mutation than hyl1-2 (Liu et al. 2012). Also , the effect of the other helicase mutant allele, dcl1-13, on miRNA production was shown to depend on the presence or absence of DRB1/HYL1 in vivo (Tagami et al. 2009). These observations indicate that efficient processing of different miRNA precursors by DCL1 have different reliance upon DRB1/HYL1 and DCL1helicase domain that potentially depends on structural determinants of the miRNA precursors.Structural determinants for efficient and accurate processing of miRNA precursors by DCL1Primary transcripts of miRNA (pri-miRNA) have a characteristic secondary structure a loop-distal stem (lower stem), a miRNA duplex, a loop-proximal stem (upper stem) and a terminal loop (Figure 3). Typical miRNA maturation from these precursors requires at least two cleavages occurring at the lower and upper stems. In animals, the single-stranded base region of the loop-distal stem is recognized by the dsRNA-binding protein DGCR8, which guides the processing center of Drosha to the worsen position, whi ch is 11 nt from the base of the stem (Han et al. 2006). However, this distance-from-base rule is not sufficient for plants because the length of the loop-distal stem of plant pri-miRNAs is highly variable (Song et al. 2010). Several structural features of pri-miRNAs that influence the activity, binding position and reign overionality of the processing by DCL1 have been elucidated genetically and biochemically (Figure 3a, b).One structural determinant lies within the loop-distal stem of pri-miRNA. For the first cleavage at the loop-distal stem, bulges and unpaired regions play a major role in the efficiency of miRNA processing. Mutant pri-miRNAs with closed bulges were processed at the correct position, but resulted in the accumulation of unprocessed pre-miRNAs in vivo, indicating that the rate of succeeding processing at the loop-proximal stem was impaired (Song et al. 2010). In pri-miR171a, which has a long loop-distal stem, the first cleavage position was determined by the dist ance from a relatively unstructured region instead of the base of the stem the conserved distance from an unstructured region of the lower stem important for miRNA processing was found to be approximately 15 nt (Figure 3a) (Mateos et al. 2010 Song et al. 2010 Werner et al. 2010). The 15-nt rule was basically reproduced in an in vitro miRNA processing system using highly purified DCL1-DRB1/HYL1-SE recombinant proteins and an false pri-miRNA substrate bearing another unstructured region in the drawn-out lower stem. In addition to the canonical processing, another type of processing occurred at 15 nt from the artificially introduced unstructured region, validating the functionality of the 15-nt rule (Song et al. 2010). The wideness of bulges and unpaired regions in the lower stem for processing by DCL1 might explain why some miRNAs with a near-perfect matched stem seem to be DCL4-dependent, rather than DCL1-dependent (Rajagopalan et al. 2006 Song et al. 2010).On the loop-proximal a nd terminal loop side, a tined terminal loop (BTL) or a large terminal loop was found to be an essential structural factor that may alter directionality of processing by DCL1 and the resultant miRNA-accumulation (Figure 3b). BTL induces unreal processing of pri-miR166c both in vivo and in vitro (Zhu et al. 2013), meaning the first cleavage of the pri-miRNA occurs in the loop-proximal stem as opposed to the normal productive processing beginning in the loop-distal stem. The molecular basis of this bidirectional processing by DCL1 was further investigated using an in vitro system that reconstitutes the DCL1-processing machinery. For this purpose, DCL1, DRB1/HYL1 and SE harboring Agrobacterium tumefaciens were co-infiltrated to Nicotiana benthamiana leaves, and the transiently expressed DCL1-DRB1/HYL1-SE complex was immunoaffinity-purified two days after infiltration (Zhu et al. 2013). The reconstituted DCL1 complex cleaves the substrate pri-miRNA 16-17 nt from the unpaired region of the lower stem, supporting previous studies (Mateos et al. 2010 Song et al. 2010 Werner et al. 2010). By disrupting one of the two RNase III domains of DCL1 alternately and using 5- or 3-end labeled pri-miR166c substrates, the bidirectional nature of both productive and abortive processing was demonstrated (Zhu et al. 2013 Figure 3b).The helicase domain of DCL1 fine-tunes the position of both productive and abortive processing by DCL1 in an ATP-dependent manner (Zhu et al. 2013). DCL1Helicase complex could not abortively process a substrate with BTL. Also, wild-type DCL1 required ATP for abortive processing, but not productive processing, indicating that the ATPase-driven helicase activity is necessary in abortive processing to unwind the structured BTL (Zhu et al. 2013 Figure 3b). In productive processing, the effect of helicase baseball swing and ATP depletion depend on the distance between the processing site and the bulge in the lower stem. Many potential byproducts of the abo rtive processing of pri-miRNA precursors with BTL can be found in publically available high-throughput small RNA sequencing data from both Arabidopsis and rice, implying that both substrate structure and the functionality of the ATPase/helicase domain of DCL1 are conserved mechanisms to regulate miRNA biogenesis in higher plants (Zhu et al. 2013).Dissecting distinct characteristics of DCL3 and DCL4 activitiesDCL4 activity requires the dsRNA-binding protein DRB4In A. thaliana, DCL2, DCL3 and DCL4 are responsible for producing various siRNAs 21-24 nt in length. The dsRNA-cleaving activities of DCL3 and DCL4 can be detected in crude extracts prepared from 2-week-old seedlings (Fukudome et al. 2011). Extracts from wild-type seedlings cleave 500-bp dsRNA substrates into 21-nt and 24-nt small RNAs. In this system, the 21-nt and 24-nt small RNA-producing activities can be attributed to DCL4 and DCL3 respectively, because the dsRNA-cleaving activity of the tally size was abolished in each of the single mutants (Fukudome et al. 2011). Also, a mutation in the dsRNA-binding protein DRB4, which interacts with DCL4 (Hiraguri et al. 2005 Nakazawa et al. 2007), abolished DCL4 activity in seedling extracts. The DCL4 activity could be further purified by immunoprecipitation with anti-DCL4 or anti-DRB4 antibodies. The immunoaffinity-purified DCL4 requires Mg2+ and ATP for its activity, and is inhibited by 200 mM NaCl. This property is similar to that of recombinant DCL1 protein (Dong et al. 2008).The DCL4 complex immunoprecipitated from the drb4-1 mutant did not show dsRNA-cleaving activity, but the addition of recombinant DRB4 protein to the complex restored the 21-nt producing activity in vitro, showing that DRB4 functions as an essential co-factor for the dsRNA-cleaving activity of DCL4 (Figure 4b). In this system, mutant DRB4 proteins harboring substitutions in the conserved amino acid residues that form a enthalpy bond with the phosphodiester backbone of dsRNA at the dsRN A-binding site (H32A in the first dsRBD and K133A in the second dsRBD of DRB4) lost their ability to interact with dsRNA and DCL4, and did not restore DCL4 activity. The second substitution (K133A) alone impaired its interaction with the C-terminal half of DCL4 containing two RNase III domains and two dsRBDs in a GST pull-down assay using recombinant proteins, but was not sufficient to block restoration of DCL4 activity when added to DCL4 immunopurified from a drb4-1 mutant extract. There might be an additional interaction surface between DCL4 and DRB4 involving dsRBD1 of DRB4 and the N-terminal half of DCL4, which contains an ATPase/DExH-box RNA-helicase domain and an RNA-binding domain (formerly known as domain of unknown quantity function DUF283 Figure 1), as their specific interaction was reported in vitro (Qin et al. 2010).Short dsRNA preference of DCL3 activity orchestrates 24-nt siRNA biogenesis in TGS pathway scratchy extracts from 2-week-old seedlings have also been used t o characterize substrate specificity of DCL3 and DCL4. Consistent with the long dsRNA preference of Drosophila Dcr1 (Bernstein et al. 2001), DCL4 preferentially cleaves longer dsRNA substrates, and is less efficient in producing 21-nt siRNAs when the substrate is shorter than 50 nt (Nagano et al. 2014). On the other hand, DCL3 activity, producing 24-nt siRNAs, favors shorter substrates such as 30 nt and 37 nt dsRNA with a 1-nt or 2-nt 3-overhang (Nagano et al. 2014). It also favors substrate dsRNA with 5-adenosine or uridine. The 24-nt small RNA produced by DCL3 has a 2-nt 3-overhang, and the cleavage follows the 5-counting rule proposed for human Dicer (Park et al. 2011). DCL3 is not reliant on ATP hydrolysis for activity, as it can still process the short dsRNA substrate in the presence of a non-hydrolyzable ATP analog, adenosine 5-O-(3-thio)triphosphate (Nagano et al. 2014). Unlike DCL4, which targets long dsRNAs such as RDR6-dependent TAS dsRNAs or exogenous viral dsRNAs in vivo (Bouche et al. 2006 Dunoyer et al. 2005 Qu et al. 2008 Yoshikawa et al. 2005), DCL3 may not need to perform a processive cleavage, which requires ATP hydrolysis, because the length of its targets allows only a single cut (Figure 4a).The DCL3 preference for short dsRNA substrate is consistent with the one precursor, one siRNA model for RNA polymerase IV ( political leader IV)-dependent 24-nt siRNA biogenesis (Blevins et al. 2015 Zhai et al. 2015). In this model, a remarkably short (30- to 40-nt) transcript with 5-adenosine is produced by Pol IV and is simultaneously converted into double-stranded form by an RNA-dependent RNA polymerase, RDR2. The short dsRNA substrate is processed into 24-nt siRNA preferentially by DCL3 due to its length specificity, facilitating the accompanying RNA-directed DNA methylation process (Blevins et al. 2015 Zhai et al. 2015). The transcription of short RNAs by Pol IV, and the length and 5-adenosine substrate preference of DCL3 might be essential mechan isms to prevent other DCLs from processing specific dsRNA substrate needed for the TGS pathway. Such coupling of RDR-Dicer-RNAi is also known in fission yeast, where a Dicer physically interacts with an RNA-dependent RNA polymerase to form coupled machinery that drives siRNA-mediated TGS (Colmenares et al. 2007).In addition, DCL3 can participate in 24-nt siRNA production from longer transcripts with aid from another RNase III enzyme, RNase III-like 2 (RTL2). As a class II RNase III enzyme, RTL2 possesses one RNase III domain and two dsRBDs, and is involved in rRNA maturation in vivo is impliedin A. thaliana (Comella et al. 2008). Recombinant RTL2 protein can cleave long dsRNA substrates into 25 bp or longer dsRNA in vitro (Kiyota et al. 2011). Recently, it has been shown that RTL2 processes a subset of Pol IV-dependent dsRNA into shorter intermediates, which are preferable for DCL3 activity in vivo (Elvira-Matelot et al. 2016). Although no direct interaction has been reported, RTL2 and DCL3 can be considered other examples of coordinated action of a dsRBD-containing protein and a Dicer in plants. DCL3 is also reported to physically interact with the dsRNA-binding protein DRB3 in the antiviral RNA-directed DNA methylation pathway (Raja et al. 2014). The function of DRB3 in DCL3 activity remains elusive.Inorganic phosphate, NaCl and KCl differentially regulate DCL3 and DCL4 activitiesIn the same assay system using crude extracts, inorganic phosphate at a physiological concentration promotes DCL3 activity but suppresses DCL4 activity toward 50-nt dsRNA substrates (Nagano et al. 2014). The differential effe